Researchers: Connie S. Alingayao and Manilyn M. Parado, SY 2007 - 2008/ Project Adviser: Mr. Darwin Q. LumosbogThis was one of the entries of the Division of Zamboanga Sibugay to the 2007 Intel Philippines Regional Science Fair. The researchers were interested on addressing the issue on Dengue infection by the mosquitoes. The students thought of a way to minimize population growth of mosquitoes by eradicating their larvae. They won third place under Cluster 1 - Life Science, Team Category.
ABSTRACT
The larvicidal property of crude aqueous Madre de cacao extract against mosquito larvae was studied. Extracts were taken from fresh young stem’s scrapings and leaves mixed with equal parts of water. 20, 15 and 15 mL of the two extracts were independently treated on the 10 mosquito wrigglers suspended in 5.0 mL rain water in separate Petri dishes. Five trials were done for all the setups. All the groups were undisturbed for 24 hours. The greatest mortality was observed in 20 and 15 mL leaf extract with both means of 9.4 (94% mortality, with n=5). Over-all results showed that the leaf extract had the most number of larvae killed with a mean of 9.00 at n=15. With a t – value of 16.508 the difference was significant, so leaf extract showed greater larvicidal activity than the stem extract. ANOVA on the comparison of larval mortality among leaf extracts resulted in an F – value of 1.500. With a significant level of .262 there was no significant difference in the number of larvae killed among the leaf extracts of different amounts. This project found out that Madre de cacao exhibits larvicidal property and that its leaf extract had a better action compared to that of its stem.
The larvicidal property of crude aqueous Madre de cacao extract against mosquito larvae was studied. Extracts were taken from fresh young stem’s scrapings and leaves mixed with equal parts of water. 20, 15 and 15 mL of the two extracts were independently treated on the 10 mosquito wrigglers suspended in 5.0 mL rain water in separate Petri dishes. Five trials were done for all the setups. All the groups were undisturbed for 24 hours. The greatest mortality was observed in 20 and 15 mL leaf extract with both means of 9.4 (94% mortality, with n=5). Over-all results showed that the leaf extract had the most number of larvae killed with a mean of 9.00 at n=15. With a t – value of 16.508 the difference was significant, so leaf extract showed greater larvicidal activity than the stem extract. ANOVA on the comparison of larval mortality among leaf extracts resulted in an F – value of 1.500. With a significant level of .262 there was no significant difference in the number of larvae killed among the leaf extracts of different amounts. This project found out that Madre de cacao exhibits larvicidal property and that its leaf extract had a better action compared to that of its stem. 
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